Short Courses > Analysis of single particles (cells)

Single Particle and Single Cell ICP-MS Theory and Applications

Presenters : Chady Stephan, Samad Bazargan, Ruth Merrifield, PerkinElmer, Shelton, CT,

This short course will cover both the fundamentals of nanoparticle detection, with Single Particle (SP) ICP-MS, and the metal content of individual cells via Single Cell (SC) ICP-MS.

SP-ICP-MS has become regularly used in metallic NP detection and quantification. The theory of SP-ICP-MS will be reviewed, focusing on the state-of-the-art innovations in hardware and software. We will then build on this to discuss the use of all matrix solution (AMS) dilution for the detection of particles in complex matrices and dual analyte SP-ICP-MS. The former allowed for the analysis of samples without the need for off line sample dilution which can rapidly transform the NPs, while the latter will allow for the quantification of two analytes in a single particle enabling compositional and particle source information. Dual analyte analysis requires a deeper understanding of the effect of transient signal times, dwell times and settling times necessary for quantitative analysis of more than one analyte per particle.

SC-ICP-MS allows for the quantification of nutrient metals as well as the uptake of metals and metal NPs into cells. We will introduce the concept of single cell-ICP-MS and discuss the encounters faced when analyzing cells compared to NPs with an in-depth focus on the necessary validation required to accurately quantify the number of particles and/or metal content in individual unicellular organism. The use of dual analysis of metals with SC-ICP-MS within cells will also be discussed.

Keywords: Single cell ICP-MS, single particle ICP-MS, bioaccumulation, nanoparticles, uptake, environment, cancer, algae

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