Short Courses > Metrology for spectrochemical measurements

Metrology for spectrochemical measurements

Presenters : Ewa Bulska, Biological and Chemical Research Centre, University of Warsaw, Poland.

Accuracy and reliability of the results of chemical measurements are of importance for both, the use of results for regulatory purposes as well as in scientific investigation. Thus, the understanding of metrological principle become a crucial knowledge for all conducting measurements with the use of atomic and molecular spectrometry. This short course will address specific issues of traceability and uncertainty of results, as the main aspect of validation of analytical procedure. Various examples of how to tailor fit for purpose calibration and the use of chemical standards as well as certified reference materials (CRMs) will be given. The use of matrix-matched CRMs, which mimic the composition of samples in respect of matrix and analyte, will be discussed focusing on atomic and molecular mass spectrometry.

The topics covered include in particular:

- the concept of traceability of chemical measurements, and the issues related to the production and certification of reference materials (RM’s)
- the use of chemical standards as well as matrix certified reference materials (CRMs)
- the fit for purpose calibration of instruments in atomic and molecular spectroscopy

Several examples covered the certification of lanthanides in plants, the elemental composition of herring, cormorant and codfish tissues as well as sediments, mercury and methylmercury in marine biota samples (scallop, oyster and clam tissues), will be discussed. In the case of molecular mass spectrometry, when LC-MS/MS is used, the strategy applied for the implementation of the standards for biologically active substances in living organisms will be described for selected proteins and doping substances. This will be exemplified by the preparation and certification of selenoproteins standards as well as hGH in human serum.

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