Short Courses > Speciation analysis

Speciation analysis: complementarity of elemental and molecular mass spectrometry in life sciences studies

Presenters : Joanna Szpunar, IPREM, CNRS, Pau, France

The development of trace inorganic analysis was a response to the increasing concerns about the role of chemical elements, even when present at low concentrations, in the living organisms. The classical approach providing informAation on the total element concentration in a bulk sample has been giving way to finer approaches including trace element spatial (imaging) or molecular resolution (speciation). In recent years, each of these approaches has grown into a separate field with a number of specific applications. The short course lecture will address the complementarity of elemental and molecular information to study the status, pathways and transformations of trace elements in biota.

The topics discussed will include:

- the concept of elemental speciation, the occurrence and classification of metal and metalloid species,
- the techniques used, the criteria of choice of a method for speciation analysis
- coupling of chromatography with ICP MS detection
- ICP MS-assisted proteomics
- electrospray MS in speciation analysis (de novo identification of organometallic species, characterization of metal complexes with peptides and proteins)
- complementarity of ICP and MALDI MS in bio-imaging
- selected case studies
- perspectives and remaining challenges

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